A B23-interacting sequence as a tool to visualize protein interactions in a cellular context

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A B23-interacting sequence as a tool to visualize protein interactions in a cellular context.

We report the characterization of a nucleolar localization sequence (NoLS) that targets the green fluorescent protein (GFP) into the granular component (GC) of nucleoli. This NoLS interacts in vitro specifically and directly with the major nucleolar protein B23 and more precisely with the region of B23 including the two acidic stretches. The affinity of NoLS for B23 is stronger than that of the...

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ژورنال

عنوان ژورنال: Journal of Cell Science

سال: 2007

ISSN: 1477-9137,0021-9533

DOI: 10.1242/jcs.03345